Cortactin anticorps (N-Term)

N° du produit ABIN2854674

Cet anticorps anti-Cortactin est un anticorps Lapin Polyclonal détectant Cortactin dans WB, IF, IHC (p) et ICC. Adapté pour Humain. Validé indépendamment pour une utilisation dans Multiplex Immunohistochemistry.

Aperçu rapide pour Cortactin anticorps (N-Term) (ABIN2854674)

Antigène: Cortactin (CTTN)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cortactin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Classe de qualité: KO Validated

Détail du produit anti-Cortactin anticorps

Épitope: N-Term

Réactivité croisée: Humain, Souris, Rat

Attributs du produit: Rabbit Polyclonal antibody to Cortactin (src substrate cortactin)
Cortactin antibody [N1], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogène: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Cortactin. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: NIH-3T3 , JC , PC-12 , mouse brain , rat brain , HeLa

Validation: KO/KD

Restrictions: For Research Use only

Independent Validation (mIHC)

Validation #104334 (Multiplex Immunohistochemistry)

by: Akoya Biosciences

No.: #104334

Date: 20.04.2021

Antigène: Cortactin

Numéro du lot: 39568

Application validée: Multiplex Immunohistochemistry

Contrôle positif:

Fresh frozen mouse olfactory bulb

Contrôle négative:

Unlabeled control (mouse fresh frozen)

Conclusion:

Passed. The anti-cortactin antibody ABIN2854674 produces high degrees of labeling in granular and external plexiform layers of the mouse olfactory bulb. Additional labeling can be observed in the neuropil of individual glomeruli which is consistent with staining of synapses and neuronal processes.

Validation Images

Murine fresh frozen coronal olfactory bulb section (Thickness = 5 µm) labeled with anti-cortactin antibody ABIN2854674 (green; conjugated to fluorophore ATTO 550). Labeling is present in high degrees of labeling in granular and external plexiform layers with additional staining observed in the glomerular neuropil regions. Grin2b and doublecortin were labeled with ABIN5611338 (red; bound to fluorophore ATTO 550) and a doublecortin antibody (blue; bound to fluorophore AF 488).

FFPE normal human cortex tissue section labeled with anti-cortactin antibody ABIN2854674 (green; bound to fluorophore ATTO 550) after EDTA antigen retrieval. MAP2 and neurofilament were additionally labeled with anti-MAP2 antibody ABIN125739 (red; bound to fluorophore ATTO 550) and anti-neurofilament antibody (blue; bound to fluorophore AF488).

Protocole

Anticorps primaire: ABIN2854674

Full Protocol:

• Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
• Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
• Conjugation of the anti-cortactin ABIN2854674 antibody to an oligo barcode used to bind oligo-conjugated fluorophore ATTO 550.

Notes:

• No signal was detected in unlabeled specimens.

• Specific staining of Cortactin was also observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.

• Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur Cortactin

Antigène: Cortactin (CTTN)

Autre désignation: cortactin

Sujet: This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.

Cellular Localization: Cytoplasm , cytoskeleton (By similarity) , Cell projection , lamellipodium (By similarity) , ruffle (By similarity)

Poids moléculaire: 62 kDa

ID gène: 2017

UniProt: Q14247

Pathways: Signalisation MAPK