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Cortactin anticorps (N-Term)

KO Validated CTTN Reactivité: Humain WB, IF, IHC (p), ICC Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN2854674
  • Antigène Voir toutes Cortactin (CTTN) Anticorps
    Cortactin (CTTN)
    Épitope
    • 15
    • 13
    • 12
    • 7
    • 7
    • 7
    • 6
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivité
    • 123
    • 60
    • 43
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 122
    • 7
    • 1
    Lapin
    Clonalité
    • 112
    • 18
    Polyclonal
    Conjugué
    • 73
    • 8
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Cortactin est non-conjugé
    Application
    • 95
    • 52
    • 51
    • 15
    • 14
    • 14
    • 14
    • 13
    • 9
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
     Réactivité croisée
    Humain, Souris, Rat
    Attributs du produit
    Rabbit Polyclonal antibody to Cortactin (src substrate cortactin)
    Cortactin antibody [N1], N-term
    Purification
    Purified by antigen-affinity chromatography.
    Classe de qualité
    KO Validated
    Immunogène
    Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Cortactin. The exact sequence is proprietary.
    Isotype
    IgG
  • Indications d'application
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
    Commentaires

    Positive Control: NIH-3T3 , JC , PC-12 , mouse brain , rat brain , HeLa

    Validation: KO/KD

    Restrictions
    For Research Use only
  • Validation #104334 (Multiplex Immunohistochemistry)
    'Independent Validation' signe
    by
    Akoya Biosciences
    No.
    #104334
    Date
    20.04.2021
    Antigène
    Cortactin
    Numéro du lot
    39568
    Application validée
    Multiplex Immunohistochemistry
    Contrôle positif

    Fresh frozen mouse olfactory bulb

    Contrôle négative

    Unlabeled control (mouse fresh frozen)

    Conclusion

    Passed. The anti-cortactin antibody ABIN2854674 produces high degrees of labeling in granular and external plexiform layers of the mouse olfactory bulb. Additional labeling can be observed in the neuropil of individual glomeruli which is consistent with staining of synapses and neuronal processes.

    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    ABIN2854674
    Anticorps secondaire
    Full Protocol
    • Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
    • Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
    • Conjugation of the anti-cortactin ABIN2854674 antibody to an oligo barcode used to bind oligo-conjugated fluorophore ATTO 550.
    Notes
    • No signal was detected in unlabeled specimens.

    • Specific staining of Cortactin was also observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.

    • Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300
    Agent conservateur
    ProClin
    Précaution d'utilisation
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Antigène
    Cortactin (CTTN)
    Autre désignation
    cortactin (CTTN Produits)
    Synonymes
    anticorps EMS1, anticorps 1110020L01Rik, anticorps Ems1, anticorps Cttnb, anticorps CG3637, anticorps Dmel\CG3637, anticorps cortactin, anticorps cttn, anticorps CTTN1, anticorps P85.25, anticorps Cttn, anticorps ems1, anticorps CTTN, anticorps cortactin, anticorps CG3637 gene product from transcript CG3637-RD, anticorps cortactin S homeolog, anticorps cortactin L homeolog, anticorps src substrate cortactin, anticorps Src substrate cortactin, anticorps CTTN, anticorps Cttn, anticorps Cortactin, anticorps cttn.S, anticorps cttn.L, anticorps cttn, anticorps CpipJ_CPIJ006351, anticorps Bm1_57220, anticorps LOC100533277
    Sujet
    This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.

    Cellular Localization: Cytoplasm , cytoskeleton (By similarity) , Cell projection , lamellipodium (By similarity) , ruffle (By similarity)
    Poids moléculaire
    62 kDa
    ID gène
    2017
    UniProt
    Q14247
    Pathways
    Signalisation MAPK
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