Retinoblastoma 1 anticorps (C-Term)

N° du produit ABIN2854790

L’anticorps Lapin Polyclonal anti-Retinoblastoma 1 a été validé pour WB, IF, IP, IHC (p) et ICC. Il convient pour détecter Retinoblastoma 1 dans des échantillons de Humain.

Aperçu rapide pour Retinoblastoma 1 anticorps (C-Term) (ABIN2854790)

Antigène: Retinoblastoma 1 (RB1)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Retinoblastoma 1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Détail du produit anti-Retinoblastoma 1 anticorps

Épitope: C-Term

Réactivité croisée: Macaque rhésus

Réactivité croisée (Details): Rhesus Monkey (100 %)

Attributs du produit: Rabbit Polyclonal antibody to Rb (retinoblastoma 1)
Rb antibody

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the C-terminus region of human Rb. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Commentaires:

Positive Control: A431 , *CL1-0 , *CL1-5 ,

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur Retinoblastoma 1

Antigène: Retinoblastoma 1 (RB1)

Autre désignation: Rb

Sujet: The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Cellular Localization: Nucleus

Poids moléculaire: 106 kDa

ID gène: 5925

Pathways: Cycle Cellulaire, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy