Calnexin anticorps (Internal Region)

N° du produit ABIN2855124

Cet anticorps anti-Calnexin est un anticorps Lapin Polyclonal détectant Calnexin dans WB, IF, ICC et IHC (p). Adapté pour Humain, Souris et Rat. Ce Primary Antibody a été cité dans 3+ publications.

Aperçu rapide pour Calnexin anticorps (Internal Region) (ABIN2855124)

Antigène: Calnexin (CANX)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Calnexin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Calnexin anticorps

Épitope: Internal Region

Réactivité croisée: Poulet, Chien, Xenopus laevis, Poisson zèbre (Danio rerio), Boeuf (Vache)

Réactivité croisée (Details): Chicken (91 %), Dog (94 %), Xenopus laevis (88 %), Zebrafish (82 %), Bovine (94 %)

Attributs du produit: Rabbit Polyclonal antibody to Calnexin (calnexin)
Calnexin antibody [N3C2], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human Calnexin. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:10000*

Commentaires:

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3 , PC-12 , Rat2

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.87 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-Calnexin (ABIN2855124)

de Jong, Murphy, Mäger, Willms, Garcia-Guerra, Gitz-Francois, Lefferts, Gupta, Steenbeek, van Rheenen, El Andaloussi, Schiffelers, Wood, Vader: "A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA." dans: Nature communications, Vol. 11, Issue 1, pp. 1113, (2020) (PubMed).

Ilahibaks, Lei, Mol, Deshantri, Jiang, Schiffelers, Vader, Sluijter: "Biofabrication of Cell-Derived Nanovesicles: A Potential Alternative to Extracellular Vesicles for Regenerative Medicine." dans: Cells, Vol. 8, Issue 12, (2020) (PubMed).

Sever, Vo, Bols, Dixon: "Rainbow trout (Oncorhynchus mykiss) contain two calnexin genes which encode distinct proteins." dans: Developmental and comparative immunology, Vol. 42, Issue 2, pp. 211-9, (2014) (PubMed).

Détails sur Calnexin

Antigène: Calnexin (CANX)

Autre désignation: Calnexin

Sujet: This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described.

Cellular Localization: Endoplasmic reticulum membrane , Melanosome

Poids moléculaire: 68 kDa

ID gène: 821

Pathways: Signalisation MAPK, Thyroid Hormone Synthesis