PHGDH anticorps (N-Term)

N° du produit ABIN2855237

L’anticorps Lapin Polyclonal anti-PHGDH a été validé pour WB, IHC (p), IF, IP, IHC (fro) et ICC. Il convient pour détecter PHGDH dans des échantillons de Humain.

Aperçu rapide pour PHGDH anticorps (N-Term) (ABIN2855237)

Antigène: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PHGDH est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Classe de qualité: KO Validated

Détail du produit anti-PHGDH anticorps

Épitope: N-Term

Réactivité croisée: Humain, Souris, Rat

Attributs du produit: Rabbit Polyclonal antibody to PHGDH (phosphoglycerate dehydrogenase)
PHGDH antibody [N1N2], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the N-terminus region of human PHGDH. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: Mouse brain , 293T , A431 , HeLaS3 , HeLa

Validation: KO/KD

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.87 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur PHGDH

Antigène: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Autre désignation: phosphoglycerate dehydrogenase

Sujet: 3-Phosphoglycerate dehydrogenase (PHGDH, EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM]

Poids moléculaire: 57 kDa

ID gène: 26227

UniProt: O43175

Pathways: Metabolism of Steroid Hormones and Vitamin D, L'effet Warburg