PD-L1 anticorps (C-Term)

N° du produit ABIN2855940

Détail du produit anti-PD-L1 anticorps

Antigène: PD-L1 (CD274 (PD-L1))

Épitope: C-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PD-L1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée: Humain

Attributs du produit: Rabbit Polyclonal antibody to PD-L1 (CD274 Molecule)
PD-L1 antibody

Purification: Purified by antigen-affinity chromatography.

Classe de qualité: KO Validated

Immunogène: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PD-L1. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: MDA-MB-231 (24μg/ml Tunicamycin treatment for 16 hr) , MDA-MB-231 , A431(100 ng/mL IFN-gamma treatment for 48 hr)

Validation: Comparison, KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.29 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7), 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-PD-L1 (ABIN2855940)

Kim, Kim, Kim, Kim, Kim: "Differential Expression and Clinicopathological Significance of HER2, Indoleamine 2,3-Dioxygenase and PD-L1 in Urothelial Carcinoma of the Bladder." dans: Journal of clinical medicine, Vol. 9, Issue 5, (2020) (PubMed).

Qin, Wang, Ye, Li, Chen, Wang, Qin, Zhang, Li, Long, Hu, Shi, Li, Zhang, Zhai, Tang, Kang, Lan, Xie, Lu, Deng: "NPM1 upregulates the transcription of PD-L1 and suppresses T cell activity in triple-negative breast cancer." dans: Nature communications, Vol. 11, Issue 1, pp. 1669, (2020) (PubMed).

Gondhowiardjo, Handoko, Adham, Rachmadi, Kodrat, Tobing, Haryoga, Dwiyono, Kristian, Mayang Permata: "Tumor microenvironment predicts local tumor extensiveness in PD-L1 positive nasopharyngeal cancer." dans: PLoS ONE, Vol. 15, Issue 3, pp. e0230449, (2020) (PubMed).

Pan, Wu, Kan, Li, Chang, Wu, Li, Ou-Yang, Hou, Yip, Luo: "Impact of FAK Expression on the Cytotoxic Effects of CIK Therapy in Triple-Negative Breast Cancer." dans: Cancers, Vol. 12, Issue 1, (2019) (PubMed).

Cha, Yang, Xia, Wei, Chan, Lim, Li, Kim, Chang, Lee, Hsu, Wang, Kuo, Chang, Hadad, Purdie, McCoy, Cai, Tu, Litton, Mittendorf, Moulder, Symmans, Thompson, Piwnica-Worms, Chen, Khoo, Hung: "Metformin Promotes Antitumor Immunity via Endoplasmic-Reticulum-Associated Degradation of PD-L1." dans: Molecular cell, Vol. 71, Issue 4, pp. 606-620.e7, (2019) (PubMed).

Buisseret, Garaud, de Wind, Van den Eynden, Boisson, Solinas, Gu-Trantien, Naveaux, Lodewyckx, Duvillier, Craciun, Veys, Larsimont, Piccart-Gebhart, Stagg, Sotiriou, Willard-Gallo: "Tumor-infiltrating lymphocyte composition, organization and PD-1/ PD-L1 expression are linked in breast cancer." dans: Oncoimmunology, Vol. 6, Issue 1, pp. e1257452, (2017) (PubMed).

Lin, Sung, Hsieh, Tsai, Lai, Yang, Shen, Chen, Lee, Yeh, Chen: "High PD-L1 Expression Correlates with Metastasis and Poor Prognosis in Oral Squamous Cell Carcinoma." dans: PLoS ONE, Vol. 10, Issue 11, pp. e0142656, (2016) (PubMed).

Détails sur PD-L1

Antigène: PD-L1 (CD274 (PD-L1))

Autre désignation: CD274 molecule (PD-L1 Produits)

Synonymes: anticorps B7-H, anticorps B7H1, anticorps PD-L1, anticorps PDCD1L1, anticorps PDCD1LG1, anticorps PDL1, anticorps A530045L16Rik, anticorps B7h1, anticorps Pdcd1l1, anticorps Pdcd1lg1, anticorps Pdl1, anticorps RGD1566211, anticorps CD274 molecule, anticorps CD274 antigen, anticorps programmed cell death 1 ligand 2, anticorps CD274, anticorps Cd274, anticorps PDCD1LG2

Sujet: Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

Cellular Localization: Isoform 1: Cell membrane, Single-pass type I membrane protein , Isoform 2: Endomembrane system, Single-pass type I membrane protein

Poids moléculaire: 33 kDa

ID gène: 29126

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints