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NAPA anticorps (Center)

NAPA Reactivité: Humain, Souris, Rat WB, IP, ICC, IF, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN2856408
  • Antigène Voir toutes NAPA Anticorps
    NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))
    Épitope
    • 15
    • 11
    • 2
    • 2
    • 2
    • 1
    Center
    Reactivité
    • 46
    • 15
    • 9
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat
    Hôte
    • 43
    • 5
    Lapin
    Clonalité
    • 41
    • 7
    Polyclonal
    Conjugué
    • 18
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp NAPA est non-conjugé
    Application
    • 33
    • 14
    • 13
    • 12
    • 10
    • 7
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
     Réactivité croisée
    Macaque rhésus, Chimpanzé
    Réactivité croisée (Details)
    Rhesus Monkey (100 %), Chimpanzee (100 %)
    Attributs du produit
    Rabbit Polyclonal antibody to alpha SNAP (N-ethylmaleimide-sensitive factor attachment protein, alpha)
    alpha SNAP antibody [N2C3]
    Purification
    Purified by antigen-affinity chromatography.
    Immunogène
    Recombinant protein encompassing a sequence within the center region of human alpha SNAP. The exact sequence is proprietary.
    Isotype
    IgG
  • Indications d'application
    Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation Assay-dependent dilution Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* ImmunoprecipitationAssay-dependent dilution Western blot1:1000-1:10000*
    Commentaires

    Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
    Agent conservateur
    Thimerosal (Merthiolate)
    Précaution d'utilisation
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Antigène
    NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))
    Autre désignation
    alpha SNAP (NAPA Produits)
    Synonymes
    anticorps SNAPA, anticorps wu:fa26c04, anticorps zgc:63906, anticorps napb, anticorps 1500039N14Rik, anticorps AW209189, anticorps RA81, anticorps SNARE, anticorps a-SNAP, anticorps hyh, anticorps alpha-SNAP, anticorps Alpha-SNAP, anticorps NSF attachment protein alpha, anticorps N-ethylmaleimide-sensitive factor attachment protein, alpha b, anticorps NSF attachment protein alpha L homeolog, anticorps N-ethylmaleimide-sensitive factor attachment protein, alpha, anticorps N-ethylmaleimide sensitive fusion protein attachment protein alpha, anticorps soluble NSF attachment protein, anticorps NAPA, anticorps napab, anticorps napa.L, anticorps napa, anticorps EHI_000300, anticorps Napa, anticorps LOC102577508
    Sujet
    The 'SNARE hypothesis' is a model explaining the process of docking and fusion of vesicles to their target membranes. According to this model, membrane proteins from the vesicle (v-SNAREs) and proteins from the target membrane (t-SNAREs) govern the specificity of vesicle targeting and docking through mutual recognition. Once the 2 classes of SNAREs bind to each other, they form a complex that recruits the general elements of the fusion apparatus, namely NSF (N-ethylmaleimide-sensitive factor) and SNAPs (soluble NSF-attachment proteins), to the site of membrane fusion, thereby forming the 20S fusion complex. Alpha- and gamma-SNAP are found in a wide range of tissues and act synergistically in intra-Golgi transport. The sequence of the predicted 295-amino acid human protein encoded by NAPA shares 37 % , 60 %, and 67 % identity with the sequences of yeast, Drosophila, and squid alpha-SNAP, respectively. Platelets contain some of the same proteins, including NSF, p115/TAP, alpha-SNAP, gamma-SNAP, and the t-SNAREs syntaxin-2 and syntaxin-4, that are used in many vesicular transport processes in other cell types. Platelet exocytosis uses a molecular mechanism similar to that used by other secretory cells, such as neurons, although the proteins used by the platelet and their modes of regulation may be quite different.

    Cellular Localization: Membrane, Peripheral membrane protein
    Poids moléculaire
    33 kDa
    ID gène
    8775
    Pathways
    Synaptic Vesicle Exocytosis, Asymmetric Protein Localization
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