FH anticorps

N° du produit ABIN2856710

L’anticorps Lapin Polyclonal anti-FH a été validé pour WB, IF, IHC (p) et ICC. Il convient pour détecter FH dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour FH anticorps (ABIN2856710)

Antigène: FH (Fumarate Hydratase (FH))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FH est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Classe de qualité: KO Validated

Détail du produit anti-FH anticorps

Réactivité croisée: Humain, Souris, Rat

Attributs du produit: Rabbit Polyclonal antibody to Fumarate hydratase (fumarate hydratase)
Fumarate hydratase antibody

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human Fumarate hydratase. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: mouse liver , rat liver , 293T

Validation: KO/KD

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.49 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-FH (ABIN2856710)

Miettinen, Felisiak-Golabek, Wasag, Chmara, Wang, Butzow, Lasota: "Fumarase-deficient Uterine Leiomyomas: An Immunohistochemical, Molecular Genetic, and Clinicopathologic Study of 86 Cases." dans: The American journal of surgical pathology, Vol. 40, Issue 12, pp. 1661-1669, (2017) (PubMed).

Détails sur FH

Antigène: FH (Fumarate Hydratase (FH))

Autre désignation: fumarate hydratase

Sujet: The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

Cellular Localization: Mitochondrion , Cytoplasm

Poids moléculaire: 55 kDa

ID gène: 2271

UniProt: P07954