Choline Acetyltransferase anticorps (N-Term) (PE)

N° du produit ABIN2869110

L’anticorps Lapin Polyclonal anti-Choline Acetyltransferase a été validé pour WB, IF et ICC. Il convient pour détecter Choline Acetyltransferase dans des échantillons de Humain.

Aperçu rapide pour Choline Acetyltransferase anticorps (N-Term) (PE) (ABIN2869110)

Antigène: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Choline Acetyltransferase est conjugé à/à la PE

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-Choline Acetyltransferase anticorps (PE)

Épitope: N-Term

Specificité: Predicted molecular weight at ~82.5 kDa. Observed molecular weights between 68-70 kDa.

Réactivité croisée: Humain, Souris

Purification: Peptide Affinity Purified

Immunogène: Synthetic peptide from the N-terminal to the mid-protein of human Choline O-Acetyltrasferase

Information d'application

Indications d'application:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

A 1:1000 dilution of ABIN2869110 was sufficient for detection of Choline Acetyltransferase on mouse brain lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur Choline Acetyltransferase

Antigène: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Autre désignation: Choline Acetyltransferase

Sujet: Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker. ChAT is a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease and Down syndrome (1-3).

ID gène: 1103

NCBI Accession: NP_001136401

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development