The mouse monoclonal antibody VIM8 recognizes human CD65, an asialo-fucoganglioside expressed on the surface of peripheral blood granulocytes (highly) and monocytes (weakly).
Réactivité croisée (Details)
Human
Purification
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Reactivité: Humain
FACS
Hôte: Souris
Monoclonal
VIM8
PE
Indications d'application
Flow cytometry: Recommended dilution: 1-5 μg/mL.
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Store at 2-8°C. Do not freeze.
Antigène
CD65
Sujet
CD65 is a fucosylated carbohydrate antigen (ceramide-dodecasaccharide, type II fucoganglioside), which serves as a ligand for CD62E (E-selectin). Its structure is Gal beta1-4 GlcNAc beta1-3 Gal beta1-4 GlcNAc (3-1 Fuc alpha) beta1-3 ceramide. Unlike CD65s, the CD65 antigen does not contain terminal sialic acid, the rest of their structure is identical. CD65 is expressed on granulocytes and monocytes and participates in cell adhesion. It has been reported as important for extravascular infiltration of acute monocytic leukemia cells.,ceramide-dodecasaccharide, type II fucoganglioside