Aperçu rapide pour CD65 anticorps (FITC) (ABIN2967177)
Antigène
CD65
Reactivité
Humain
Hôte
Souris
Clonalité
Monoclonal
Conjugué
FITC
Application
Flow Cytometry (FACS)
Clone
VIM8
Fonction
Anti-Hu CD65 FITC
Réactivité croisée (Details)
Human
Attributs du produit
The mouse monoclonal antibody VIM8 recognizes human CD65, an asialo-fucoganglioside expressed on the surface of peripheral blood granulocytes (highly) and monocytes (weakly).
Purification
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Restrictions
For Research Use only
Format
Liquid
Buffer
Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Do not freeze,Protect from prolonged exposure to light
Stock
4 °C
Stockage commentaire
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Antigène
CD65
Sujet
CD65 is a fucosylated carbohydrate antigen (ceramide-dodecasaccharide, type II fucoganglioside), which serves as a ligand for CD62E (E-selectin). Its structure is Gal beta1-4 GlcNAc beta1-3 Gal beta1-4 GlcNAc (3-1 Fuc alpha) beta1-3 ceramide. Unlike CD65s, the CD65 antigen does not contain terminal sialic acid, the rest of their structure is identical. CD65 is expressed on granulocytes and monocytes and participates in cell adhesion. It has been reported as important for extravascular infiltration of acute monocytic leukemia cells.,ceramide-dodecasaccharide, type II fucoganglioside