Cet anticorps anti-Alkaline Phosphatase Polyclonal Lapin conjugué à HRP (ABIN288216) détecte spécifiquement Alkaline Phosphatase dans WB et ELISA.
L’anticorps est réactif avec des échantillons de E. coli.
ALP
Reactivité: Boeuf (Vache)
WB, IHC, ICC, IP
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: 1:4,000-1:20,000, WB: 1:500-1:2,000 Optimal conditions should be determined by the investigator.
Restrictions
For Research Use only
Format
Lyophilized
Concentration
Lot specific
Buffer
Lyophilized from 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, 10 mg/mL BSA, and 0.01 % gentamycin sulfate. DO NOT add Sodium azide. Immunoglobulin and protease free.
Conseil sur la manipulation
Avoid repeated freeze/thaw cycles. Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. Dilute only prior to immediate use.
Stock
4 °C/-20 °C
Stockage commentaire
Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
Antigène
Alkaline Phosphatase (ALP)
Autre désignation
Alkaline Phosphatase
Sujet
Alkaline phosphatase is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase.