Histone H2B anticorps (AA 111-125)

N° du produit ABIN291581

L’anticorps anti-Histone H2B Polyclonal Lapin est utilisé pour la détection de Histone H2B dans des échantillons de Humain, Souris, Rat, Boeuf (Vache), Poulet, Poisson zèbre (Danio rerio), Insectes et Drosophila melanogaster. Il a été validé pour WB, IHC et IHC (p).

Aperçu rapide pour Histone H2B anticorps (AA 111-125) (ABIN291581)

Antigène: Histone H2B

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Poulet, Poisson zèbre (Danio rerio), Insectes, Drosophila melanogaster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone H2B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Histone H2B anticorps

Épitope: AA 111-125

Specificité: A 15 kD band is observed. This is routinely quality controlled in human lysates.

Homologie: Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine, Chicken, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Nematode (100%).

Purification: Protein G purified

Immunogène: This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acid residues 111-125 AVSEGTKAVTKYTSSof human Histone H2B, GenBank no. CAI19747.1. This peptide sequence is 100% conserved between multiple species including human, mouse, rat, dog, chimpanzee, cow, chicken, duck, zebrafish, and drosophila. Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine, Chicken, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Nematode (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: avoid freeze thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Détails sur Histone H2B

Antigène: Histone H2B