NOTCH4 anticorps (AA 1778-2003)

N° du produit ABIN291848

L’anticorps Chèvre Polyclonal anti-NOTCH4 a été validé pour WB, ELISA et ICC. Il convient pour détecter NOTCH4 dans des échantillons de Humain.

Aperçu rapide pour NOTCH4 anticorps (AA 1778-2003) (ABIN291848)

Antigène: NOTCH4 (Notch 4 (NOTCH4))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp NOTCH4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Détail du produit anti-NOTCH4 anticorps

Épitope: AA 1778-2003

Specificité: Recognizes human Notch4.

Purification: Affinity purified

Immunogène: E. coli-derived rhNotch-4 (aa1778-2003).

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human Notch4 intracellular domain. The detection limit for rhNotch4 intracellular domain is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL to detect human Notch4 in serum starved HUVEC cells stimulated with Jagged 1. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4?C followed by Rhodamine Red-coupled anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human Notch4 intracellular domain. The detection limit for rhNotch4 intracellular domain is approximately 0.3 ng/well.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Détails sur NOTCH4

Antigène: NOTCH4 (Notch 4 (NOTCH4))

Autre désignation: NOTCH4

Sujet: Name/Gene ID: NOTCH4

Synonyms: NOTCH4, HNotch4, Notch (Drosophila) homolog 4, Notch homolog 4 (Drosophila), Notch homolog 4, INT3, Notch 4

ID gène: 4855

UniProt: Q99466

Pathways: Signalisation Notch