GLI3 anticorps

N° du produit ABIN292775

L’anticorps Chèvre Polyclonal anti-GLI3 a été validé pour WB, ELISA et ICC. Il convient pour détecter GLI3 dans des échantillons de Humain et Souris.

Aperçu rapide pour GLI3 anticorps (ABIN292775)

Antigène: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GLI3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Détail du produit anti-GLI3 anticorps

Specificité: Recognizes human and mouse GLI-3 in the applications listed. In direct ELISAs and Western Blots, the antibody shows less than 1 % cross-reactivity with rhGLI-1 and rhGLI-2.

Purification: Affinity purified

Immunogène: E. coli-derived rhGLI-3.

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Immunocytochemistry, and Western Blot. Western Blot: 0.1-0.2 μg/mL. Used in conjunction with the appropriate secondary reagents, detects human and mouse GLI-3. The detection limit for rhGLI-3 and rmGLI-3 is approximately 5 ng/lane under non- reducing and reducing conditions. Immunocytochemistry: 10 μg/mL detects human GLI-3 in HeLa cells. Cells were fixed with PBS containing 4 % paraformaldehyde and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA. After blocking, cells were incubated with diluted primary antibody followed by NorthernLights 557-coupled anti-goat IgG at room temperature in the dark. Between each step, cells were washed with PBS containing BSA. Direct ELISA: 0.5-1.0 μg/mL. Used in conjunction the appropriate secondary reagents, detects human and mouse GLI-3. The detection limit for rhGLI-3 and rmGLI-3 is approximately 1 ng/well.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Détails sur GLI3

Antigène: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Autre désignation: GLI3

Sujet: Name/Gene ID: GLI3

Synonyms: GLI3, ACLS, GCPS, GLI-Kruppel family member GLI3, GLI3 full length protein, GLI3-190, GLI family zinc finger 3, Gli-3, GLI3FL, PAP-A, PAPA1, PHS, PPDIV, Transcriptional activator GLI3, PAPA, PAPB, GLI3 form of 190 kDa, Oncogene GLI3, Zinc finger protein GLI3

ID gène: 2737

UniProt: P10071

Pathways: Signalisation Hedgehog