MEK1 + MEK2 anticorps (pSer217, Ser221)

N° du produit ABIN294413

L’anticorps anti-MEK1 + MEK2 Polyclonal Lapin est utilisé pour la détection de MEK1 + MEK2 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB et IP.

Aperçu rapide pour MEK1 + MEK2 anticorps (pSer217, Ser221) (ABIN294413)

Antigène: MEK1 + MEK2

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MEK1 + MEK2 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Détail du produit anti-MEK1 + MEK2 anticorps

Épitope: pSer217, Ser221

Specificité: Detects endogenous levels of MEK1/2 only when activated by phosphorylation at Ser217/221. Does not cross-react with related kinases including activated SEK (MKK4), MKK3 or MKK6. It will also react with MEK1/2 singly phosphorylated at Ser217, but not with MEK1/2 singly phosphorylated at Ser221. Species cross-reactivity: Human, mouse and rat.

Purification: Protein A purified

Immunogène: Synthetic phosphopeptide corresponding to residues around Ser217/221 of human MEK1/2 (KLH coupled). MW: 45kD.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IP (1:50), WB (1:1000)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur MEK1 + MEK2

Antigène: MEK1 + MEK2

Autre désignation: MEK1 + MEK2

Sujet: This antibody detects: MEK1 + MEK2