SMAD6 anticorps (AA 85-99)

N° du produit ABIN295868

L’anticorps anti-SMAD6 Polyclonal Lapin est utilisé pour la détection de SMAD6 dans des échantillons de Humain et Porc. Il a été validé pour WB, IHC et IHC (p).

Aperçu rapide pour SMAD6 anticorps (AA 85-99) (ABIN295868)

Antigène: SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))

Reactivité: Humain, Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SMAD6 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-SMAD6 anticorps

Épitope: AA 85-99

Specificité: A 52 kD band is observed.

Homologie: Percent identity by BLAST analysis: Human, Pig (100%) Dog, Bovine, Opossum (93%) Gibbon, Marmoset, Rat (87%) Elephant (80%).

Purification: Protein G purified

Immunogène: This antibody was developed against synthetic peptides corresponding to amino acids 85-99 (RAGGPPRPMSEPGAG)and amino acids 372-388 (NLEQRSESVRRTRSKIG)of human SMAD6 (GenBank Accession No. AAH12986.1). These peptide sequences are specific to SMAD6, and have high homology to SMAD6in multiple species. Percent identity by BLAST analysis: Human, Pig (100%), Dog, Bovine, Opossum (93%), Gibbon, Marmoset, Rat (87%), Elephant (80%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: avoid freeze thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Détails sur SMAD6

Antigène: SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))

Autre désignation: SMAD6

Sujet: Name/Gene ID: SMAD6

Synonyms: SMAD6, AOVD2, HSMAD6, HsT17432, SMAD 6, MADH7, Mothers against DPP homolog 6, SMAD family member 6, MAD homolog 6, MADH6

ID gène: 4091

UniProt: O43541

Pathways: Chromatin Binding