ABCB1 anticorps

N° du produit ABIN296626

Cet anticorps Souris Monoclonal détecte spécifiquement ABCB1 dans IHC, IHC (p), FACS, IP, IF et IHC (fro). Il présente une réactivité envers Humain et Primate.

Aperçu rapide pour ABCB1 anticorps (ABIN296626)

Antigène: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Reactivité: Humain, Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ABCB1 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: UIC2

Détail du produit anti-ABCB1 anticorps

Specificité: Recognizes an extracellular conformational epitope of CD243, also known as MDR1 (multi-drug resistance protein 1) and Pgp (P-glycoprotein), a transmembrane protein and member of the ABC transporter (ATP-binding cassette) family, which acts as an active efflux pump for a diverse range of lipophillic compounds. CD243 is expressed at low levels in the cell membrane of peripheral blood leucocytes, and constitutively expressed on the apical plasma membrane of excretory epithelial cells of the kidney, liver, brain and small intestine. Strong inhibitor of CD243-mediated efflux and of the resistance of MDR cells to CD243 transported cytotoxic drugs. Can be used in a shift assay to selectively demonstrate the expression and functional activity of CD243 in a target cell. Does not cross-react with mitochondrial pyruvate carboxylase.

Aucune reactivité croisée: Souris, Rat (Rattus)

Purification: Affinity purified

Immunogène: Mouse Balb/c 3T3 fibroblasts transfected with human CD243 cDNA.

Type of Immunogen: Cells

Isotype: IgG2a

Information d'application

Indications d'application: Approved: Flo (1:50 - 1:200), IF, IHC, IHC-Fr, IHC-P, IP

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry: This product may benefit from antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer  pH 6.0 is recommended for this purpose (3-4). Histology Positive Control Tissue: Human kidney.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Short term: -20°C
Long term: -20°C.

Détails sur ABCB1

Antigène: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Autre désignation: ABCB1 / MDR1 / P Glycoprotein

Sujet: Name/Gene ID: ABCB1
Subfamily: ATP-binding cassette - ABCB/MDR
Family: Transporter

Synonyms: ABCB1, ABC20, Abcb1b, CLCS, Colchicin sensitivity, CD243, Doxorubicin resistance, IBD13, gp170, MDR1, Multidrug resistance protein 1, P glycoprotein, P-glycoprotein 1, P-GP, CD243 antigen, PGY1

ID gène: 5243

UniProt: P08183