DKK1 anticorps (AA 250-262)

N° du produit ABIN296913

Cet anticorps anti-DKK1 Polyclonal Chèvre (ABIN296913) détecte spécifiquement DKK1 dans WB, ELISA, IHC et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Souris, Chien, Boeuf (Vache), Cheval, Chévre, Porc et Singe.

Aperçu rapide pour DKK1 anticorps (AA 250-262) (ABIN296913)

Antigène: DKK1 (Dickkopf Homolog 1 (DKK1))

Reactivité: Humain, Souris, Chien, Boeuf (Vache), Cheval, Chévre, Porc, Singe

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp DKK1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-DKK1 anticorps

Épitope: AA 250-262

Specificité: Human DKK1.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Bovine, Dog, Goat, Elephant, Panda, Horse, Pig (100%) Gibbon, Rat, Bat, Hamster, Platypus (92%) Rabbit (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-DHHQASNSSRLHT from the C-terminus of human DKK1 (NP_036374.1). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Bovine, Dog, Goat, Elephant, Panda, Horse, Pig (100%), Gibbon, Rat, Bat, Hamster, Platypus (92%), Rabbit (85%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 μg/mL), Peptide-ELISA (1:128000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur DKK1

Antigène: DKK1 (Dickkopf Homolog 1 (DKK1))

Autre désignation: DKK1

Sujet: Name/Gene ID: DKK1

Synonyms: DKK1, Dickkopf-1 like, DKK-1, Dickkopf-1, Dickkopf-related protein 1, Dickkopf related protein-1, HDkk-1, Hdkk1, Dickkopf-like protein 1, SK

ID gène: 22943

NCBI Accession: NP_036374

UniProt: O94907

Pathways: Signalisation WNT, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation