AHNAK anticorps (N-Term)

N° du produit ABIN302097

Cité dans 1 publication. Cet anticorps anti-AHNAK Monoclonal Souris (Clone EM-09) (ABIN302097) détecte spécifiquement AHNAK dans WB, IP, ICC et IHC (fx). L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour AHNAK anticorps (N-Term) (ABIN302097)

Antigène: AHNAK (AHNAK Nucleoprotein (AHNAK))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp AHNAK est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Fixed) (IHC (fx))

Clone: EM-09

Détail du produit anti-AHNAK anticorps

Épitope: N-Term

Fonction: Anti-AHNAK1 Purified

Réactivité croisée (Details): Human, Mouse

Attributs du produit: The mouse monoclonal antibody EM-09 reacts with AHNAK1, a 700 kDa multi-functional adaptor protein expressed mainly in epithelial cell, various types of muscle cells and immune cells.

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Bacterially expressed fragment of N-terminal domain of human AHNAK1.

Isotype: IgG1

Information d'application

Indications d'application: Immunocytochemistry: Positive control: HeLa human cervix carcinoma cell line, permeabilization is required.
Western blotting: Recommended dilution: 1 μg/mL, non-reducing conditions

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-AHNAK (ABIN302097)

Johnson, Zhang, Adams, Phillips, Freitas, Froehner, Green-Church, Montanaro: "Proteomic analysis reveals new cardiac-specific dystrophin-associated proteins." dans: PLoS ONE, Vol. 7, Issue 8, pp. e43515, (2012) (PubMed).

Détails sur AHNAK

Antigène: AHNAK (AHNAK Nucleoprotein (AHNAK))

Autre désignation: AHNAK1

Sujet: AHNAK1 nucleoprotein,AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear, it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.,Desmoyokin

ID gène: 79026

UniProt: Q09666