Neurofilament anticorps (Extracellular Domain)

N° du produit ABIN3023931

L’anticorps Souris Monoclonal anti-Neurofilament a été validé pour IF et IHC (p). Il convient pour détecter Neurofilament dans des échantillons de Humain.

Aperçu rapide pour Neurofilament anticorps (Extracellular Domain) (ABIN3023931)

Antigène: Neurofilament

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Neurofilament est non-conjugé

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM563

Détail du produit anti-Neurofilament anticorps

Épitope: Extracellular Domain

Purification: Protein G affinity chromatography

Immunogène: A membrane preparation of small cell lung carcinoma was used as the immunogen for this anti-CD56 antibody. This mAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM.

Isotype: IgG1 kappa

Information d'application

Indications d'application: The optimal dilution of the anti-CD56 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the anti-CD56 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Neurofilament

Antigène: Neurofilament

Sujet: Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135  kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.