Neurofilament anticorps

N° du produit ABIN3025341

L’anticorps anti-Neurofilament Monoclonal Souris est utilisé pour la détection de Neurofilament dans des échantillons de Humain, Rat et Souris. Il a été validé pour FACS, WB, IF et IHC (p).

Aperçu rapide pour Neurofilament anticorps (ABIN3025341)

Antigène: Neurofilament

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Neurofilament est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Classe de qualité: Carrier-free

Clone: NF421

Détail du produit anti-Neurofilament anticorps

Fonction: Neurofilament Antibody / Heavy (azide and preservative free)

Purification: Protein G purified Neurofilament antibody

Immunogène: Human recombinant Neurofilament protein was used as the immunogen. (1)

Isotype: IgG1, kappa

Information d'application

Indications d'application: The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the Neurofilament antibody to be titered for optimal performance.

1. The NF421 antibody is specific to the heavy subunit.
2. FFPE tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Neurofilament antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur Neurofilament

Antigène: Neurofilament

Sujet: Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). The fourth neurofilament subunit is alpha internexin in the central nervous system and peripherin in the peripheral nervous system.