Neurofilament anticorps

N° du produit ABIN3023931

L’anticorps anti-Neurofilament Monoclonal Souris est utilisé pour la détection de Neurofilament dans des échantillons de Humain. Il a été validé pour WB et IHC (p).

Aperçu rapide pour Neurofilament anticorps (ABIN3023931)

Antigène: Neurofilament

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Neurofilament est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Classe de qualité: Carrier-free

Clone: SPM563

Détail du produit anti-Neurofilament anticorps

Fonction: Anti-Neurofilament Antibody (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogène: Human recombinant protein was used as the immunogen for this anti-Neurofilament antibody.

Isotype: IgG1, kappa

Information d'application

Indications d'application: The optimal dilution of the anti-Neurofilament antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the anti-Neurofilament antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur Neurofilament

Antigène: Neurofilament

Sujet: This mAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.

UniProt: P12036