CLIP anticorps (AA 25-39)

N° du produit ABIN3024112

Cet anticorps anti-CLIP Monoclonal Souris (Clone ADCT1-1) (ABIN3024112) détecte spécifiquement CLIP dans IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour CLIP anticorps (AA 25-39) (ABIN3024112)

Antigène: CLIP (Corticotropin-Like Intermediate Peptide (CLIP))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CLIP est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: ADCT1-1

Détail du produit anti-CLIP anticorps

Épitope: AA 25-39

Fonction: ACTH Antibody / CLIP

Séquence: NGAEDESAEA FPLEF

Purification: Protein G affinity chromatography

Immunogène: Amino acids 25-39 (NGAEDESAEAFPLEF) of Adrenocorticotropic hormone (CLIP sequence) were used as the immunogen for this ACTH antibody.

Isotype: IgG1, kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the ACTH antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue requires boiling sections in 10 mM Citrate Buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the ACTH antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur CLIP

Antigène: CLIP (Corticotropin-Like Intermediate Peptide (CLIP))

Autre désignation: ACTH / CLIP

Sujet: ACTH (or Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This mAb is specific to CLIP (aa 25-39) and does not react with Synacthen (aa 1-24). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.