CD8 alpha anticorps (C-Term, Cytoplasmic Domain)

N° du produit ABIN3024498

L’anticorps anti-CD8 alpha Monoclonal Souris est utilisé pour la détection de CD8 alpha dans des échantillons de Humain. Il a été validé pour IHC (p).

Aperçu rapide pour CD8 alpha anticorps (C-Term, Cytoplasmic Domain) (ABIN3024498)

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8 alpha est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Classe de qualité: Carrier-free

Clone: C8-468-C8-144B

Détail du produit anti-CD8 alpha anticorps

Épitope: C-Term, Cytoplasmic Domain

Fonction: CD8a Antibody Cocktail (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogène: Human recombinant protein (C8/468) and 13 amino acid peptide from the C-terminal cytoplasmic domain of the alpha chain of human CD8 (C8/144B) were used as the immunogens for this CD8a antibody cocktail.

Isotype: IgG1, kappa

Information d'application

Indications d'application: The optimal dilution of the CD8a antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the CD8a antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur CD8 alpha

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Autre désignation: CD8a

Sujet: CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8a/b) or as a homodimer (CD8a/a). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.

UniProt: P01732

Pathways: TCR Signaling