Cytokeratin 8/18 anticorps (full length)

N° du produit ABIN3024667

Cet anticorps anti-Cytokeratin 8/18 Monoclonal Souris (Clone KRT8-803-KRT18-835) (ABIN3024667) détecte spécifiquement Cytokeratin 8/18 dans IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Cytokeratin 8/18 anticorps (full length) (ABIN3024667)

Antigène: Cytokeratin 8/18

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 8/18 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: KRT8-803-KRT18-835

Détail du produit anti-Cytokeratin 8/18 anticorps

Épitope: full length

Fonction: Cytokeratin 8/18 Antibody Cocktail

Purification: Protein G affinity chromatography

Immunogène: Recombinant full-length human KRT8 protein (KRT8/803) and KRT18 protein (KRT18/835) were used as the immunogen for the Cytokeratin 8/18 antibody cocktail.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal dilution of the Cytokeratin 8/18 antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Cytokeratin 8/18 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur Cytokeratin 8/18

Antigène: Cytokeratin 8/18

Sujet: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This mAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained. Immuno-histochemical staining with this mAb is indistinguishable from that obtained with monoclonal antibody 5D3.

UniProt: P05787