Cytokeratin 8/18 anticorps

N° du produit ABIN3024678

L’anticorps anti-Cytokeratin 8/18 Monoclonal Souris est utilisé pour la détection de Cytokeratin 8/18 dans des échantillons de Humain. Il a été validé pour IF, WB, FACS et IHC (p).

Aperçu rapide pour Cytokeratin 8/18 anticorps (ABIN3024678)

Antigène: Cytokeratin 8/18

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 8/18 est non-conjugé

Application: Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Classe de qualité: Carrier-free

Clone: C-51

Détail du produit anti-Cytokeratin 8/18 anticorps

Fonction: Cytokeratin 8/18 Antibody (azide and preservative free)

Aucune reactivité croisée: Souris, Rat (Rattus), Furet, Lapin, Âne, Poulet, Xenopus laevis

Purification: Protein G affinity chromatography

Immunogène: A cytoskeleton preparation from HeLa cells was used as the immunogen for the Cytokeratin 8/18 antibody.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal dilution of the Cytokeratin 8/18 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Cytokeratin 8/18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur Cytokeratin 8/18

Antigène: Cytokeratin 8/18

Sujet: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This mAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained.

UniProt: P05787