FBXO5 anticorps (AA 1-250)

N° du produit ABIN3024717

L’anticorps Souris Monoclonal anti-FBXO5 a été validé pour WB, IF, FACS et IHC (p). Il convient pour détecter FBXO5 dans des échantillons de Humain.

Aperçu rapide pour FBXO5 anticorps (AA 1-250) (ABIN3024717)

Antigène: FBXO5 (F-Box Protein 5 (FBXO5))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FBXO5 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: EMI1-1176

Détail du produit anti-FBXO5 anticorps

Épitope: AA 1-250

Purification: Protein G affinity chromatography

Immunogène: A recombinant fragment (203 amino acid residues between aa 1-250) from the human protein was used as the immunogen for the EMI1 antibody.

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal dilution of the EMI1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the EMI1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur FBXO5

Antigène: FBXO5 (F-Box Protein 5 (FBXO5))

Autre désignation: EMI1

Sujet: Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of EMI1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Pathways: Mitotic G1-G1/S Phases