Fascin anticorps

N° du produit ABIN3024845

Cet anticorps Souris Monoclonal détecte spécifiquement Fascin dans FACS, IF et IHC (p). Il présente une réactivité envers Humain et Rat.

Aperçu rapide pour Fascin anticorps (ABIN3024845)

Antigène: Fascin (FSCN1)

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Fascin est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: FSCN1-418

Détail du produit anti-Fascin anticorps

Purification: Protein G affinity chromatography

Immunogène: Full length recombinant human protein was used as the immunogen for the Fascin antibody.

Isotype: IgG2b kappa

Information d'application

Indications d'application: Optimal dilution of the Fascin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1  mM EDTA,  pH 8.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistology (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Fascin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Fascin

Antigène: Fascin (FSCN1)

Autre désignation: Fascin

Sujet: Fascin/FSCN1 binds beta-catenin and colocalizes with it at the leading edges and borders of epithelial and endothelial cells. The role of FSCN1 in regulating cytoskeletal structures for the maintenance of cell adhesion, coordinating motility and invasion through interactions with signalling pathways is an active area of research especially from the cancer biology perspective. It localizes to actin-rich protrusions at the cell surface called filopodia. FSCN1 localizes to invadopodia, membrane protrusions formed at the adherent cell surface that facilitate extracellular matrix (ECM) invasion, this provide a potential molecular mechanism for how fascin increases the invasiveness of cancer cells since fascin expression is upregulated in a spectrum of cancers. [Wiki]