Calponin anticorps

N° du produit ABIN3025302

L’anticorps Souris Monoclonal anti-Calponin a été validé pour IHC (p), IF et FACS. Il convient pour détecter Calponin dans des échantillons de Humain et Rat.

Aperçu rapide pour Calponin anticorps (ABIN3025302)

Antigène: Calponin

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Calponin est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: CNN1-832-CALP

Détail du produit anti-Calponin anticorps

Purification: Protein G affinity chromatography

Immunogène: Recombinant full-length human protein (CNN1/832) and crude human uterus extract (CALP) were used as the immunogen for the Calponin antibody cocktail.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the Calponin antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Calponin antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Calponin

Antigène: Calponin

Sujet: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34  kDa form and a 29  kDa form. Expression of the 29  kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this mAb reacts with only the 34  kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.