HCAM anticorps

N° du produit ABIN3025614

L’anticorps anti-HCAM Monoclonal Souris est utilisé pour la détection de HCAM dans des échantillons de Humain et Souris. Il a été validé pour IHC (p).

Aperçu rapide pour HCAM anticorps (ABIN3025614)

Antigène: HCAM

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: HCAM-1097

Détail du produit

Fonction: HCAM Antibody

Purification: Protein G affinity chromatography

Immunogène: Recombinant human protein was used as the immunogen for the HCAM antibody.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal dilution of the HCAM antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the HCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détail du antigène

Antigène: HCAM

Sujet: Recognizes a cell surface glycoprotein of 80-95 kDa (CD44/HCAM) on lymphocytes, monocytes, and granulocytes. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95 kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44/HCAM immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

UniProt: P16070