HCAM anticorps

N° du produit ABIN6251853

Cet anticorps Souris Monoclonal détecte spécifiquement HCAM dans WB, IHC, FACS, IF et ELISA. Il présente une réactivité avec des échantillons de Humain, Babouin et Green Monkey.

Aperçu rapide pour HCAM anticorps (ABIN6251853)

Antigène: HCAM

Reactivité: Humain, Babouin, Green Monkey

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA

Clone: 156-3C11

Détail du produit

Purification: Protein G affinity chromatography

Immunogène: Stimulated human leukocytes were used as the immunogen for this CD44 / HCAM antibody.

Isotype: IgG2a kappa

Information d'application

Indications d'application: ELISA: order BSA-free format for coating

Flow Cytometry: 0.5-1 μg/million cells

IF: 0.5-1 μg/mL

WB: 0.5-1 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCAM antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détail du antigène

Antigène: HCAM

Sujet: This antibody recognizes a cell surface glycoprotein of 80-95  kDa, called CD44, or HCAM, on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95  kDa or hematopoietic variant (CD44-s). Higher molecular weight isoforms are described in epithelial cells (CD44-v), which are believed to function in intercellular adhesion and stromal binding. HCAM antibody immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

ID gène: 960

UniProt: P16070