NUMA1 anticorps

N° du produit ABIN3025683

L’anticorps Souris Monoclonal anti-NUMA1 a été validé pour WB, IF et IHC (p). Il convient pour détecter NUMA1 dans des échantillons de Humain.

Aperçu rapide pour NUMA1 anticorps (ABIN3025683)

Antigène: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NUMA1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: A73-B-D12

Détail du produit anti-NUMA1 anticorps

Purification: PEG precipitation

Immunogène: Colon carcinoma 174T cells were used as the immunogen for the NuMA antibody.

Isotype: IgM kappa

Information d'application

Indications d'application: Optimal dilution of the NuMA antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.\. Western blot: 1-2 μg/mL,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the NuMA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur NUMA1

Antigène: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Autre désignation: NuMA

Sujet: Recognizes a phosphorylated protein of 228  kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200  kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, M Phase