BCL2L1 anticorps (AA 3-14)

N° du produit ABIN3025808

Cet anticorps Souris Monoclonal détecte spécifiquement BCL2L1 dans WB, IF, FACS et IHC (p). Il présente une réactivité envers Humain, Souris, Rat et Porc.

Aperçu rapide pour BCL2L1 anticorps (AA 3-14) (ABIN3025808)

Antigène: BCL2L1 (BCL2-Like 1 (BCL2L1))

Reactivité: Humain, Souris, Rat, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BCL2L1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: BX006-2H12

Détail du produit anti-BCL2L1 anticorps

Épitope: AA 3-14

Purification: Protein G affinity chromatography

Immunogène: Amino acids 3-14 (QSNRELVVDFLS) from the human protein were used as the immunogen for this Bcl-X antibody.

Isotype: IgG2a

Information d'application

Indications d'application: The optimal dilution of the Bcl-X antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Bcl-X antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur BCL2L1

Antigène: BCL2L1 (BCL2-Like 1 (BCL2L1))

Autre désignation: Bcl-X

Sujet: Recognizes a protein of 27  kDa, identified as the Bcl-X protein. This mAb cocktail shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This mAb reacts with both Bcl-XS and Bcl-XL proteins.

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling