Metabotropic Glutamate Receptor 5 anticorps (Extracellular Domain, N-Term)

N° du produit ABIN302583

Cet anticorps anti-Metabotropic Glutamate Receptor 5 est un anticorps Lapin Polyclonal détectant Metabotropic Glutamate Receptor 5 dans IHC (p). Adapté pour Rat, Humain et Souris.

Aperçu rapide pour Metabotropic Glutamate Receptor 5 anticorps (Extracellular Domain, N-Term) (ABIN302583)

Antigène: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Reactivité: Rat, Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Metabotropic Glutamate Receptor 5 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Metabotropic Glutamate Receptor 5 anticorps

Épitope: Extracellular Domain, N-Term

Specificité: This antibody detects Metabotropic Glutamate Receptor 5 (GRM5) at N-terminal extracellular domain.

Purification: Immunoaffinity chromatography

Immunogène: GRM5 antibody was raised against synthetic 17 amino acid peptide from N-terminal extracellular domain of Human GRM5 / MGLUR5.

Information d'application

Indications d'application: Immunohistochemistry on paraffin sections (5.6 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline containing 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C for longer.

Détails sur Metabotropic Glutamate Receptor 5

Antigène: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Autre désignation: mGluR5 / GRM5

Sujet: Glutamate receptors constitute the principal excitatory neurotransmitter receptors in brain. Two classes of glutamate receptors exist: Ionotropic receptors, and metabotropic receptors (mGluRs). Ionotropic glutamate receptors are oligomeric complexes of various subunits (GluR1 7, NMDA1 3, KAI 2) which comprise ligand gated calcium channels. Metabotropic glutamate receptors are G protein coupled receptors that contain 7 membrane spanning domains and a large extracellular N terminal region. When activated, they can activate phopholipases or adenylylcyclase, depending on the neuron. The group I receptors mGluR1 and mGluR5 activate phospholipase C. By screening a human brain cDNA library with a rat mGluR1 alpha cDNA under low stringency conditions, Minakami et al. (1993) and Minakami et al. (1994) isolated human cDNAs encoding mGluR5. Minakami et al. (1993) identified a longer isoform of mGluR5 (mGluR5B).Synonyms: GPRC1E, MGLUR5, Metabotropic glutamate receptor 5

ID gène: 2915

NCBI Accession: NP_000833

UniProt: P41594

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity