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MFGE8 anticorps

MFGE8 Reactivité: Humain WB, FACS, IF, IHC (p) Hôte: Souris Monoclonal SPM291 unconjugated
N° du produit ABIN3025860
  • Antigène Voir toutes MFGE8 Anticorps
    MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))
    Reactivité
    • 62
    • 10
    • 4
    • 3
    Humain
    Hôte
    • 45
    • 21
    • 4
    • 1
    Souris
    Clonalité
    • 49
    • 22
    Monoclonal
    Conjugué
    • 44
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp MFGE8 est non-conjugé
    Application
    • 60
    • 24
    • 21
    • 19
    • 13
    • 13
    • 11
    • 11
    • 10
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purification
    Protein G affinity chromatography
    Immunogène
    A human milk fat globule membrane preparation was used as the immunogen for the MFG-E8 antibody.
    Clone
    SPM291
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product MFGE8 Anticorps primaire
  • Indications d'application
    Optimal dilution of the MFG-E8 antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Agent conservateur
    Azide free
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store the MFG-E8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Antigène
    MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))
    Autre désignation
    MFG-E8 (MFGE8 Produits)
    Synonymes
    anticorps BA46, anticorps EDIL1, anticorps HMFG, anticorps HsT19888, anticorps MFG-E8, anticorps MFGM, anticorps OAcGD3S, anticorps SED1, anticorps SPAG10, anticorps hP47, anticorps ba46, anticorps edil1, anticorps oacgd3s, anticorps MGC75581, anticorps hst19888, anticorps MGC145109, anticorps MFGE8, anticorps mfge8, anticorps zgc:154157, anticorps MGC99228, anticorps AA408458, anticorps AI325141, anticorps Mfgm, anticorps P47, anticorps lactadherin, anticorps AGS, anticorps MFGME8, anticorps MFGMP-E8, anticorps im:6895406, anticorps mfge8l, anticorps zgc:110846, anticorps milk fat globule-EGF factor 8 protein, anticorps milk fat globule-EGF factor 8 protein b, anticorps milk fat globule-EGF factor 8 protein L homeolog, anticorps milk fat globule-EGF factor 8 protein a, anticorps MFGE8, anticorps mfge8, anticorps mfge8b, anticorps mfge8.L, anticorps Mfge8, anticorps mfge8a
    Sujet
    Recognizes a protein of 40-45  kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin or MFG-E8. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.
    Pathways
    SARS-CoV-2 Protein Interactome
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