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Recombinant Keratin Acidic (AE1) anticorps

AE1 Reactivité: Humain IF, IHC (p) Hôte: Lapin Monoclonal RMAK1-1 unconjugated Recombinant Antibody
N° du produit ABIN3026306
  • Antigène Voir toutes Keratin Acidic (AE1) Anticorps
    Keratin Acidic (AE1)
    Type d'anticorp
    Recombinant Antibody
    Reactivité
    • 31
    • 11
    • 9
    • 9
    • 9
    • 7
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    Humain
    Hôte
    • 18
    • 10
    • 3
    Lapin
    Clonalité
    • 31
    Monoclonal
    Conjugué
    • 14
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Inconjugué
    Application
    • 24
    • 24
    • 23
    • 20
    • 11
    • 3
    • 3
    • 2
    Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purification
    Protein A affinity chromatography
    Immunogène
    An amino acid sequence common to acidic/type I keratins was used as the immunogen for the Acidic Cytokeratin antibody.
    Clone
    RMAK1-1
    Isotype
    IgG kappa
    Top Product
    Discover our top product AE1 Anticorps primaire
  • Indications d'application
    Titering of the Acidic Cytokeratin antibody may be required for optimal performance.

    1. This antibody will detect cytokeratins -10, -14, -15, -16, -19.
    2. FFPE testing is enhanced by boiling sections in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.\. Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    0.2 mg/mL
    Buffer
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store the Acidic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Antigène
    Keratin Acidic (AE1)
    Autre désignation
    Acidic Cytokeratin (AE1 Produits)
    Sujet
    There are two types of cytokeratins/keratins/CKs: the acidic type I cytokeratins and the basic or neutral type II cytokeratins. The subsets of cytokeratins which an epithelial cell expresses depends mainly on the type of epithelium, the moment in the course of terminal differentiation and the stage of development. Thus this specific keratin fingerprint allows the classification of all epithelia upon their keratin expression profile. Furthermore this applies also to the malignant counterparts of the epithelia (carcinomas), as the keratin profile tends to remain constant when an epithelium undergoes malignant transformation. The main clinical implication is that the study of the keratin profile by immunohistochemistry techniques is a tool of immense value widely used for tumor diagnosis and characterization in surgical pathology. [Wiki]
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