CD45RO anticorps

N° du produit ABIN3026460

L’anticorps anti-CD45RO Monoclonal Souris est utilisé pour la détection de CD45RO dans des échantillons de Humain. Il a été validé pour FACS, IF et IHC (p).

Aperçu rapide pour CD45RO anticorps (ABIN3026460)

Antigène: CD45RO

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD45RO est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Classe de qualité: Carrier-free

Clone: UCHL-1

Détail du produit anti-CD45RO anticorps

Fonction: CD45RO Antibody (azide and preservative free)

Attributs du produit: This antibody recognizes a 180-185 kDa protein, identified as isoform of leukocyte common antigen (CD45) called CD45RO (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. CD45RO is found on mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. The CD45RO antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, antibody to CD45RO is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

Purification: Protein G affinity chromatography

Immunogène: Cultured human T-cells from an IL-2-dependent T-cell line (CA1) were used as the immunogen for the CD45RO antibody.

Isotype: IgG2a, kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD45RO antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the CD45RO antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur CD45RO

Antigène: CD45RO

Autre désignation: CD45RO

Sujet: This antibody recognizes a 180-185 kDa protein, identified as isoform of leukocyte common antigen (CD45) called CD45RO (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. CD45RO is found on mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. The CD45RO antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, antibody to CD45RO is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.