CA9 anticorps

N° du produit ABIN3026518

Cet anticorps anti-CA9 Monoclonal Souris (Clone 66-4-C2) (ABIN3026518) détecte spécifiquement CA9 dans WB, FACS, IF et IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour CA9 anticorps (ABIN3026518)

Antigène: CA9 (Carbonic Anhydrase IX (CA9))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CA9 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Classe de qualité: Carrier-free

Clone: 66-4-C2

Détail du produit anti-CA9 anticorps

Fonction: CAIX Antibody / Carbonic Anhydrase IX (azide and preservative free)

Attributs du produit: This antibody recognizes a glycoprotein of ~200 kDa, identified as renal cell carcinoma protein, carbonic anhydrase IX / CAIX, or gp200. Its epitope resides in the carbohydrate domain of the protein. The CAIX antibody shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, CAIX is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, it is expressed by 93 % of primary and 84 % of metastatic renal cell carcinomas. This CAIX antibody may be useful in the investigations of carcinomas of proximal nephrogenic differentiation, especially those showing tubular differentiation.

Purification: Protein G affinity chromatography

Immunogène: The microsomal fraction of human renal cortical tissue homogenate was used as the immunogen for this Carbonic Anhydrase IX / CAIX antibody.

Isotype: IgG2b, kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CAIX antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the CAIX antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur CA9

Antigène: CA9 (Carbonic Anhydrase IX (CA9))

Autre désignation: CAIX

Sujet: This antibody recognizes a glycoprotein of ~200 kDa, identified as renal cell carcinoma protein, carbonic anhydrase IX / CAIX, or gp200. Its epitope resides in the carbohydrate domain of the protein. The CAIX antibody shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, CAIX is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, it is expressed by 93 % of primary and 84 % of metastatic renal cell carcinomas. This CAIX antibody may be useful in the investigations of carcinomas of proximal nephrogenic differentiation, especially those showing tubular differentiation.