CD68 anticorps

N° du produit ABIN3026529

L’anticorps Souris Monoclonal anti-CD68 a été validé pour FACS, IF et IHC (p). Il convient pour détecter CD68 dans des échantillons de Humain, Singe, Cat et Lapin.

Aperçu rapide pour CD68 anticorps (ABIN3026529)

Antigène: CD68 (CD68 Molecule (CD68))

Reactivité: Humain, Singe, Cat, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD68 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM130

Détail du produit anti-CD68 anticorps

Aucune reactivité croisée: Porc, Chien, Poulet

Attributs du produit: This antibody recognizes a glycoprotein of 110 kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

Purification: Protein G affinity chromatography

Immunogène: A human alveolar macrophage subcellular fraction was used as the immunogen for this anti-CD68 antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: The optimal dilution of the anti-CD68 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the anti-CD68 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur CD68

Antigène: CD68 (CD68 Molecule (CD68))

Autre désignation: CD68

Sujet: This antibody recognizes a glycoprotein of 110  kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.