CD35 anticorps

N° du produit ABIN3026591

Cet anticorps Souris Monoclonal détecte spécifiquement CD35 dans FACS, IF et IHC (p). Il présente une réactivité envers Humain, Babouin, Cynomolgus et Macaque rhésus.

Aperçu rapide pour CD35 anticorps (ABIN3026591)

Antigène: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reactivité: Humain, Babouin, Cynomolgus, Macaque rhésus

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD35 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: E11

Détail du produit anti-CD35 anticorps

Attributs du produit: This antibody recognizes a protein of 210-220 kDa, which is identified as the complement receptor 1 (CR1), or CD35. The antibody is specific for a site on CD35 that is distal from the C3b-binding site, so that it is unable to block CD35 activity. The CD35 antibody is highly specific and shows no cross-reaction with CR2. The primary function of CD35 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This antibody labels follicular dendritic cells and follicular dendritic cell sarcoma.

Purification: Protein G affinity chromatography

Immunogène: Intact human monocytes were used as the immunogen for this CD35 antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the CD35 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur CD35

Antigène: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Autre désignation: CD35

Sujet: This antibody recognizes a protein of 210-220  kDa, which is identified as the complement receptor 1 (CR1), or CD35. The antibody is specific for a site on CD35 that is distal from the C3b-binding site, so that it is unable to block CD35 activity. The CD35 antibody is highly specific and shows no cross-reaction with CR2. The primary function of CD35 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This antibody labels follicular dendritic cells and follicular dendritic cell sarcoma.

ID gène: 1378

Pathways: Système du Complément, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity