BCL2L1 anticorps

N° du produit ABIN3026614

Cet anticorps anti-BCL2L1 est un anticorps Souris Monoclonal détectant BCL2L1 dans WB, IF, FACS et IHC (p). Adapté pour Humain, Souris, Rat et Porc.

Aperçu rapide pour BCL2L1 anticorps (ABIN3026614)

Antigène: BCL2L1 (BCL2-Like 1 (BCL2L1))

Reactivité: Humain, Souris, Rat, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BCL2L1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: BX006

Détail du produit anti-BCL2L1 anticorps

Attributs du produit: This antibody recognizes a protein of 27 kDa, identified as Bcl-X. The mAb shows no cross-reaction with Bcl2 alpha or Bax. Bcl-X has two isoforms, XL (long), a 241 amino acid protein which suppresses cell death, and XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl2.

Purification: Protein G affinity chromatography

Immunogène: Amino acids QSNRELVVDFLS (3-14) of the human protien were used as the immunogen for this Bcl-X antibody (2).

Isotype: IgG2a

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bcl-X antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1  mM EDTA buffer,  pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. This antibody reacts with both BclXS and BclXL.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (3)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Bcl-X antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur BCL2L1

Antigène: BCL2L1 (BCL2-Like 1 (BCL2L1))

Autre désignation: Bcl-X

Sujet: This antibody recognizes a protein of 27  kDa, identified as Bcl-X. The mAb shows no cross-reaction with Bcl2 alpha or Bax. Bcl-X has two isoforms, XL (long), a 241 amino acid protein which suppresses cell death, and XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl2.

ID gène: 598

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling