CD147 anticorps

N° du produit ABIN3026721

L’anticorps Souris Monoclonal anti-CD147 a été validé pour FACS, IF, IHC (p) et InhA. Il convient pour détecter CD147 dans des échantillons de Humain.

Aperçu rapide pour CD147 anticorps (ABIN3026721)

Antigène: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD147 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Inhibition Assay (InhA)

Clone: 8D6

Détail du produit anti-CD147 anticorps

Attributs du produit: This antibody recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. CD147 stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.

Purification: Protein G purified CD147 antibody

Immunogène: Human T cell line Molt-13 was used as the immunogen for this CD147 antibody.

Isotype: IgG1

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD147 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Functional Activity: Inhibits homotypic aggregation and adhesion to matrix proteins: order BSA-free antibody format,FACS: 0.5-1 μg/million cells,IF: 1:100-1:200,IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the CD147 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur CD147

Antigène: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Autre désignation: CD147

Sujet: This antibody recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. CD147 stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.

ID gène: 682

Pathways: S100 Proteins