MUC5AC anticorps

N° du produit ABIN3026776

Cet anticorps anti-MUC5AC est un anticorps Souris Monoclonal détectant MUC5AC dans IF, FACS et IHC (p). Adapté pour Humain, Souris, Rat, Singe, Cat, Lapin, Poulet, Porc et Hedgehog.

Aperçu rapide pour MUC5AC anticorps (ABIN3026776)

Antigène: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Reactivité: Humain, Souris, Rat, Singe, Cat, Lapin, Poulet, Porc, Hedgehog

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MUC5AC est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 45M1

Détail du produit anti-MUC5AC anticorps

Aucune reactivité croisée: Boeuf (Vache)

Attributs du produit: This antibody recognizes the peptide core of gastric mucin M1 (>1,000 kDa), now identified as Mucin-5AC or MUC5AC. The MUC5AC epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. MUC5AC antibody reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.

Purification: Protein G affinity chromatography

Immunogène: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient was used as the immunogen for this MUC5AC antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC5AC antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the MUC5AC antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur MUC5AC

Antigène: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Autre désignation: MUC5AC Mucin-5AC

Sujet: This antibody recognizes the peptide core of gastric mucin M1 (>1,000  kDa), now identified as Mucin-5AC or MUC5AC. The MUC5AC epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. MUC5AC antibody reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.

ID gène: 4586