Fc gamma RII (CD32) anticorps

N° du produit ABIN3026914

Cet anticorps anti-Fc gamma RII Monoclonal Souris (Clone 7-3) (ABIN3026914) détecte spécifiquement Fc gamma RII dans IF et Func. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Fc gamma RII (CD32) anticorps (ABIN3026914)

Antigène: Fc gamma RII (CD32)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunofluorescence (IF), Functional Studies (Func)

Classe de qualité: Carrier-free

Clone: 7-3

Détail du produit

Fonction: CD32 Antibody / FCGR2A (azide and preservative free)

Attributs du produit: This mAb reacts with a CD32 (FcgRII) epitope (cluster-4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (FcgRII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.

Purification: Protein G affinity chromatography

Immunogène: K562 and FcgRII+L cells were used as the immunogen for the CD32 antibody.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal dilution of the CD32 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the CD32 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détail du antigène

Antigène: Fc gamma RII (CD32)

Autre désignation: CD32

Sujet: This mAb reacts with a CD32 (FcgRII) epitope (cluster-4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (FcgRII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.

UniProt: P12318