Topoisomerase II alpha anticorps

N° du produit ABIN3027012

L’anticorps Souris Monoclonal anti-Topoisomerase II alpha a été validé pour WB, IF, FACS et IHC (p). Il convient pour détecter Topoisomerase II alpha dans des échantillons de Humain.

Aperçu rapide pour Topoisomerase II alpha anticorps (ABIN3027012)

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Topoisomerase II alpha est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: TPM2A-1

Détail du produit anti-Topoisomerase II alpha anticorps

Attributs du produit: This mAb recognizes a 170 kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

Purification: Protein G affinity chromatography

Immunogène: Human partial recombinant protein was used as the immunogen for this TOP2A antibody.

Isotype: IgG2b kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue requires boiling sections in 10  mM citrate buffer,  pH 6, for 10-20 min followed by cooling at RT for 20 min.\. FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL,Western blot: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL (1)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the TOP2A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Topoisomerase II alpha

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Autre désignation: TOP2A

Sujet: This mAb recognizes a 170  kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

ID gène: 7153

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases