BUB3 anticorps (Middle Region)

N° du produit ABIN3030213

Détail du produit anti-BUB3 anticorps

Antigène: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Épitope: Middle Region

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BUB3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Antigen affinity

Immunogène: An amino acid sequence from the middle region of human Bub3 (DHQLKMHDLNTDQEN) was used as the immunogen for this Bub3 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Information d'application

Indications d'application: The stated application concentrations are suggested starting amounts. Titration of the Bub3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Stockage

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Stock: -20 °C

Stockage commentaire: After reconstitution, the Bub3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Détails sur BUB3

Antigène: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Autre désignation: Bub3 (BUB3 Produits)

Sujet: Budding uninhibited by benzimidazoles 3 is a protein that in humans is encoded by the BUB3 gene. It is a protein involved with the regulation of the Spindle Assembly Checkpoint (SAC), though Bub3 is non-essential in yeast, it is essential in higher eukaryotes. As one of the checkpoint proteins, it delays the irreversible onset of anaphase through direction of kinetochore localization during prometaphase to achieve biorentation. Bub3 directs the localization of Bub1 at the kinetochore in order to activate the SAC. It also acts as a regulator in that it affects binding of Mad3 to Mad2.

UniProt: O43684

Pathways: Maintenance of Protein Location