GLI3 anticorps (N-Term)

N° du produit ABIN3031041

Cet anticorps anti-GLI3 est un anticorps Lapin Polyclonal détectant GLI3 dans WB. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour GLI3 anticorps (N-Term) (ABIN3031041)

Antigène: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GLI3 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-GLI3 anticorps

Épitope: N-Term

Purification: Antigen affinity

Immunogène: An amino acid sequence from the N-terminus of human Gli-kruppel family member 3 (SNEDESPGQTYHRERRNA) was used as the immunogen for this Gli3 antibody.

Isotype: IgG

Information d'application

Indications d'application: The stated application concentrations are suggested starting amounts. Titration of the Gli3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. There are two isoforms of Gli-kruppel family member 3: the 170-190KD full length form and a truncated ~80KD form. This antibody will detect both, depending on the testing material.\. Western blot: 0.5-1 μg/mL (1)

Restrictions: For Research Use only

Stockage

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Stock: -20 °C

Stockage commentaire: After reconstitution, the Gli3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Détails sur GLI3

Antigène: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Autre désignation: Gli3

Sujet: Gli-kruppel family member 3 is a member of the zinc finger family related to Kruppel, a protein family that is known to regulate development in Drosophila. The gene is expressed as an 8.5-kb mRNA in tissues such as testis, myometrium, placenta, and lung, and the protein product (relative molecular mass, 190,000D) shows sequence-specific DNA binding. Gli3 is homologous to the Drosophila cubitus interruptus (ci) gene product(Ci), which regulates the Patched (pct), Gooseberry (gsb), and Decapentaplegic (dpp) genes. PKA-dependent processing of vertebrate Gli3 in developing limb generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein. The high relative abundance and potency of Gli3 repressor suggested specialization of the protein and its products for negative hedgehog pathway regulation.

UniProt: P10071

Pathways: Signalisation Hedgehog