POLK anticorps (AA 817-830)

N° du produit ABIN303130

Détail du produit anti-POLK anticorps

Antigène: POLK (Polymerase (DNA Directed) kappa (POLK))

Épitope: AA 817-830

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp POLK est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Purification: Immunoaffinity Chromatography.

Immunogène: Synthetic peptide corresponding to Amino acids 817-830 of Human POLK (polymerase (DNA directed) kappa).

Information d'application

Indications d'application: Immunohistochemistry on Paraffin Sections (5 μg/mL). Immunofluorescence. Western Blot (1/500-1/3000). ELISA (1/2000-1/10000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.8 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at-20 °C. Dilute only prior to immediate use.

Détails sur POLK

Antigène: POLK (Polymerase (DNA Directed) kappa (POLK))

Autre désignation: DNA Polymerase kappa (POLK Produits)

Synonymes: anticorps DINB1, anticorps DINP, anticorps POLQ, anticorps Dinb1, anticorps POLK, anticorps si:ch211-254o18.3, anticorps DNA polymerase kappa, anticorps polymerase (DNA directed) kappa, anticorps polymerase (DNA directed), kappa, anticorps polymerase (DNA directed) kappa L homeolog, anticorps POLK, anticorps Polk, anticorps polk, anticorps Tb11.01.0080, anticorps Tb11.01.0010, anticorps Tb11.01.0020, anticorps Tb11.01.0030, anticorps Tb11.01.0040, anticorps Tb11.01.0050, anticorps Tb11.12.0001, anticorps Tb11.12.0002, anticorps Tb11.12.0003, anticorps polk.L, anticorps polk-1

Sujet: POLK (also known as DNA polymerase kappa, DINB protein and DINP) is involved in DNA repair. POLK plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. POLK lacks 3'-5' proofreading exonuclease activity. POLK is a nuclear protein that binds to REV1L and PCNA. POLK is detected throughout the nucleus and at replication foci. Multiple alternative splice variants have been detected for this protein. POLK is found at low levels in testis, spleen, prostate and ovary and at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.Synonyms: DINB protein, DINB1, DINP, POLK

ID gène: 51426

NCBI Accession: NP_057302

UniProt: Q9UBT6

Pathways: Réparation de l'ADN