PKM2 anticorps (N-Term)

N° du produit ABIN3032236

Cet anticorps Lapin Polyclonal détecte spécifiquement PKM2 dans WB. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour PKM2 anticorps (N-Term) (ABIN3032236)

Antigène: PKM2 (Pyruvate Kinase M2 (PKM2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PKM2 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-PKM2 anticorps

Épitope: N-Term

Purification: Antigen affinity

Immunogène: An amino acid sequence from the N-terminus of human PKM2 (SHGTHEYHAETIKNV) was used as the immunogen for this PKM2 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Information d'application

Indications d'application: The stated application concentrations are suggested starting amounts. Titration of the PKM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Stockage

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Stock: -20 °C

Stockage commentaire: After reconstitution, the PKM2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Détails sur PKM2

Antigène: PKM2 (Pyruvate Kinase M2 (PKM2))

Autre désignation: PKM2

Sujet: Pyruvate Kinase, Muscle, also known as PK3 or PKM2, is an enzyme that in humans is encoded by the PKM gene. The activity of pyruvate kinase subtype M2 is increased by fructose 1, 6-bisphosphate(Fru-1, 6-P2). By in situ hybridization, Popescu and Cheng (1990) mapped the gene to 15q24-q25. Ashizawa et al.(1991) manipulated the intracellular Fru-1, 6-P2 concentration in several mammalian cell lines, including human, by varying the glucose concentration in the media. Using a novel proteomic screen for phosphotyrosine-binding proteins, Christofk et al.(2008) observed that PKM2 binds directly and selectively to tyrosine-phosphorylated peptides.

UniProt: P14618

Pathways: L'effet Warburg